bc0601018_si_001.pdf (712.37 kB)
Bionanoconjugation via Click Chemistry: The Creation of Functional Hybrids of Lipases and Gold Nanoparticles
journal contribution
posted on 2006-11-15, 00:00 authored by Jennifer L. Brennan, Nikos S. Hatzakis, T. Robert Tshikhudo, Valdemaras Razumas, Shamkant Patkar, Jesper Vind, Allan Svendsen, Roeland J. M. Nolte, Alan E. Rowan, Mathias BrustA simple and versatile method for the preparation of functional enzyme−gold nanoparticle conjugates using
“click” chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized
Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under
retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric
lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are
attached to each nanoparticle.