Bionanoconjugation via Click Chemistry: The Creation of Functional Hybrids of Lipases and Gold Nanoparticles
journal contributionposted on 2006-11-15, 00:00 authored by Jennifer L. Brennan, Nikos S. Hatzakis, T. Robert Tshikhudo, Valdemaras Razumas, Shamkant Patkar, Jesper Vind, Allan Svendsen, Roeland J. M. Nolte, Alan E. Rowan, Mathias Brust
A simple and versatile method for the preparation of functional enzyme−gold nanoparticle conjugates using “click” chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.