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Biomimicked and CPMV-Imprinted Hollow Porous Zinc Phosphate Nanocapsules and Their Therapeutic Efficiency
journal contribution
posted on 2020-09-07, 04:48 authored by Koushi Kumar, Pradip PaikHollow
zinc phosphate nanocapsules (hZPNCs) are an alloplastic
biomaterial that has been synthesized to deliver chemotherapeutic
drugs in a sustained manner. A very simple one-pot synthesis approach
has been employed to synthesize hZPNCs by using cowpea mosaic virus
(CPMV) in the presence of phosphate buffer (PBS) (0.01 M PBS, pH ∼7.2)
with zinc acetate precursor. The synthesis mechanism of hZPNCs relies
on the basis of biomineralization, where the precursor molecules initiate
mineralization with the help of amino acid residues present on the
CPMV capsid. The synthesized hollow nanocapsules were of diameter
∼50–60 nm and porous shell with thickness of ∼4
nm. The cavity performed as a reservoir for the anticancer drugs (DOX
and IM). The release kinetic studies show the positive aspect of hZPNCs
to be labeled as drug delivery cargo for sustained delivery. In vitro
cytotoxic studies of hZPNCs and hZPNCs-chemo drugs on HEK293, HEPG2,
and K562 cells were performed. The cytotoxic studies show that hZPNCs-DOX
and hZPNCs-IM arrest the cell cycle of carcinoma cells (HEPG2 and
K562 cells) at relatively low IC50 and that the inhibition
efficiency is dosage dependent. Furthermore, through HRTEM, in vitro
cellular interactions of carcinoma cells with hZPNCs and chemo drug-loaded
hZPNCs were confirmed by the cryo-sectioning of cells before and after
the incubation. These studies revealed the likely endocytic pathway
for the nanocapsules entering the cell and executing the specific
action of delivering the anticancer drugs. Together, these results
reveal the hZPNCs as potential sustained drug delivery agents.