posted on 2017-08-02, 00:00authored byAnna-Lena Winkler, Meike Koenig, Alexander Welle, Vanessa Trouillet, Domenic Kratzer, Christoph Hussal, Joerg Lahann, Cornelia Lee-Thedieck
We
report the chemical vapor deposition (CVD) of dual-functional
polymer films for the specific and orthogonal immobilization of two
biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide)
that govern the fate of hematopoietic stem and progenitor cells. The
composition of the CVD polymer and thus the biomolecule ratio can
be tailored to investigate and optimize the influence of the relative
surface concentrations of biomolecules on stem cell behavior. Prior
to cell experiments, all surfaces were characterized by infrared reflection
adsorption spectroscopy, time-of-flight secondary ion mass spectrometry,
and X-ray photoelectron spectroscopy to confirm the presence of both
biomolecules. In a proof-of-principle stem cell culture study, we
show that all polymer surfaces are cytocompatible and that the proliferation
of the hematopoietic stem and progenitor cells is predominantly influenced
by the surface concentration of immobilized DLL1.