posted on 2015-07-08, 00:00authored byCan Liu, Jiping Sheng, Lin Chen, Yanyan Zheng, David Yue Wei Lee, Yang Yang, Mingshuang Xu, Lin Shen
Bacillus subtilis strain B154, isolated
from Agaricus bisporus mushroom compost
infected by red bread mold, exhibited antagonistic activities against Neurospora sitophila. Antifungal activity against
phytopathogenic fungi was also observed. The maximum antifungal activity
was reached during the stationary phase. This antifungal activity
was stable over a wide pH and temperature range and was not affected
by proteases. Assay of antifungal activity in vitro indicated that
a purified antifungal substance could strongly inhibit mycelia growth
and spore germination of N. sitophila. In addition, treatment with strain B154 in A. bisporus mushroom compost infected with N. sitophila significantly increased the yield of bisporus mushrooms.
Ultraviolet scan spectroscopy, tricine sodium dodecyl sulfate-polyacrylamide
gel electrophoresis, matrix-associated laser desorption ionization
time-of-flight mass spectrometry, and electrospray ionization tandem
mass spectrometry analyses revealed a molecular weight consistent
with 1498.7633 Da. The antifungal compound might belong to a new type
of lipopeptide fengycin.