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Biocompatible ELR-Based Polyplexes Coated with MUC1 Specific Aptamers and Targeted for Breast Cancer Gene Therapy
journal contribution
posted on 2016-01-27, 00:00 authored by Maria
J. Piña, Alessandra Girotti, Mercedes Santos, J. Carlos Rodríguez-Cabello, F. Javier AriasThe search for new and biocompatible
materials with high potential
for improvement is a challenge in gene delivery applications. A cell
type specific vector made of elastin-like recombinamer (ELR) and aptamers
has been specifically designed for the intracellular delivery of therapeutic
material for breast cancer therapy. A lysine-enriched ELR was constructed
and complexed with plasmid DNA to give positively charged and stable
polyplexes. Physical characterization of these polyplexes showed a
particle size of around 140 nm and a zeta potential of approximately
+40 mV. The incorporation of MUC1-specific aptamers into the polyplexes
resulted in a slight decrease in zeta potential but increased cell
transfection specificity for MCF-7 breast cancer cells with respect
to a MUC1-negative tumor line. After showing the transfection ability
of this aptamer-ELR vector which is facilitated mainly by macropinocytosis
uptake, we demonstrated its application for suicide gene therapy using
a plasmid containing the gene of the toxin PAP-S. The strategy developed
in this work about using ELR as polymeric vector and aptamers as supplier
of specificity to deliver therapeutic material into MUC1-positive
breast cancer cells shows promising potential and continues paving
the way for ELRs in the biomedical field.
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Keywords
breast cancer therapyELRtransfection abilitygene delivery applicationsintracellular deliveryvectorplasmid DNA140 nmPhysical characterizationBreast Cancer Gene TherapyThe searchMUC 1cell typezetabiocompatible materialsparticle sizemacropinocytosis uptakepolyplexecell transfection specificitysuicide gene therapyMCFaptamer
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