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Biocatalytic Separation of N-7/N-9 Guanine Nucleosides

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journal contribution
posted on 19.11.2010, 00:00 by Sunil K. Singh, Vivek K. Sharma, Carl E. Olsen, Jesper Wengel, Virinder S. Parmar, Ashok K. Prasad
Vorbrüggen coupling of trimethylsilylated 2-N-isobutanoylguanine with peracetylated pentofuranose derivatives generally gives inseparable N-7/N-9 glycosyl mixtures. We have shown that the two isomers can be separated biocatalytically by Novozyme-435-mediated selective deacetylation of the 5′-O-acetyl group of peracetylated N-9 guanine nucleosides.