Bioaccumulation of CdTe Quantum Dots in a Freshwater Alga Ochromonas danica: A Kinetics Study
journal contributionposted on 2013-09-17, 00:00 authored by Ying Wang, Ai-Jun Miao, Jun Luo, Zhong-Bo Wei, Jun-Jie Zhu, Liu-Yan Yang
The bioaccumulation kinetics of thioglycolic acid stabilized CdTe quantum dots (TGA-CdTe-QDs) in a freshwater alga Ochromonas danica was comprehensively investigated. Their photoluminescence (PL) was determined by flow cytometry. Its cellular intensity increased hyperbolically with exposure time suggesting real internalization of TGA-CdTe-QDs. This hypothesis was evidenced by the nanoparticle uptake experiment with heat-killed or cold-treated cells and by their localization in the vacuoles. TGA-CdTe-QD accumulation could further be well simulated by a biokinetic model used previously for conventional pollutants. Moreover, macropinocytosis was the main route for their internalization. As limited by their diffusion from the bulk medium to the cell surface, TGA-CdTe-QD uptake rate increased proportionally with their ambient concentration. Quick elimination in the PL of cellular TGA-CdTe-QDs was also observed. Such diminishment resulted mainly from their surface modification by vacuolar biomolecules, considering that these nanoparticles remained mostly undissolved and their expulsion out of the cells was slow. Despite the significant uptake of TGA-CdTe-QDs, they had no direct acute effects on O. danica. Overall, the above research shed new light on nanoparticle bioaccumulation study and would further improve our understanding about their environmental behavior, effects and fate.
internalizationSuch diminishmentthioglycolic acidalga Ochromonas danicaexposure timeambient concentrationbulk mediumsurface modificationCdTe Quantum Dotsvacuolar biomoleculesflow cytometryKinetics StudyThe bioaccumulation kineticsbiokinetic modelPLQuick eliminationFreshwater Alga Ochromonas danicacell surfaceCdTe quantum dotsnanoparticle uptake experimentnanoparticle bioaccumulation study