Bimetallic CoMoO₄ Nanozymes Enhanced Luminol Chemiluminescence for the Detection of Dopamine

Transition-metal-based nanozymes show great potential for chemiluminescence (CL) detection due to their stability, excellent catalytic properties, good biocompatibility, and facile synthesis. In this study, bimetallic CoMoO₄ nanorods with high peroxidase activity were synthesized using a hydrothermal method, followed by calcination treatment. The as-synthesized CoMoO₄ nanorods were utilized as catalysts for CL for the first time. They enhance the CL intensity of the luminol/hydrogen peroxide system by nearly 750-fold. Furthermore, a sensitive and rapid platform for dopamine detection was established based on the quenching effect of dopamine on the CL signal of this system. The proposed CL method for dopamine exhibited a linear range of 10–500 nM and a detection limit of 1.98 nM. Moreover, dopamine in serum samples was successfully measured using a standard addition method, showing a satisfactory recovery of 97.2–104.3%. This work provides robust support for the development of efficient and sensitive platforms for CL detection.