posted on 2015-12-17, 05:47authored byMatthew
D. Lew, W. E. Moerner
Many single nanoemitters such as
fluorescent molecules produce
dipole radiation that leads to systematic position errors in both
particle tracking and super-resolution microscopy. Via vectorial diffraction
equations and simulations, we show that imaging only azimuthally polarized
light in the microscope naturally avoids emission from the z-component of the transition dipole moment, resulting in
negligible localization errors for all emitter orientations and degrees
of objective lens misfocus. Furthermore, localization accuracy is
maintained even in the presence of aberrations resulting from imaging
in mismatched media.