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Automated Enrichment of Sulfanilamide in Milk Matrices by Utilization of Aptamer-Linked Magnetic Particles
journal contribution
posted on 2016-11-09, 00:00 authored by Christin Fischer, Constanze Kallinich, Sven Klockmann, Jil Schrader, Markus FischerThe
present work demonstrates the first automated enrichment approach
for antibiotics in milk using specific DNA aptamers. First, aptamers
toward the antibiotic sulfanilamide were selected and characterized
regarding their dissociation constants and specificity toward relevant
antibiotics via fluorescence assay and LC-MS/MS detection. The performed
enrichment was automated using the KingFisherDuo and compared to a
manual approach. Verifying the functionality, trapping was realized
in different milk matrices: (i) 0.3% fat milk, (ii) 1.5% fat milk,
(iii) 3.5% fat milk, and (iv) 0.3% fat cocoa milk drink. Enrichment
factors up to 8-fold could be achieved. Furthermore, it could be shown
that novel implementation of a magnetic separator increases the reproducibility
and reduces the hands-on time from approximately half a day to 30
min.