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Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity

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journal contribution
posted on 16.12.2015 by Richard S. Gaster, Drew A. Hall, Shan X. Wang
We report an autoassembly protein array capable of rapidly screening for aberrant antibody−antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.