Herein,
a Au-Au/IrO2 nanocomposite with tandem enzyme-mimicking
activity was innovatively synthesized, which can show outstanding
glucose oxidase (GOx)-like activity and peroxidase-like activity simultaneously
under neutral conditions. Moreover, a Au-Au/IrO2@Cu(PABA)
reactor was prepared via encapsulation of the Au-Au/IrO2 nanocomposite in a Cu(PABA) metal organic framework. The reactor
not only exhibits excellent organic solvent stability, acid resistance,
and reusability but also displays better cascade reaction catalytic
efficiency (kcat/Km = 148.86 min–1 mM–1) than the natural free enzyme system (GOx/HRP) (kcat/Km = 98.20 min–1 mM–1) and Au-Au/IrO2 nanocomposite
(kcat/Km =
135.24 min–1 mM–1). In addition,
it is found that the reactor can catalyze glucose or dissolved oxygen
to produce active oxygen species (ROS) including HO, 1O2, and O2–· through
its enzyme-mimicking activity. Finally, the novel reactor was successfully
used in organic dye degradation and antibacterial application. The
results show that it can effectively degrade methyl orange, methylene
blue, and rhodamine B, which all can reach a degradation rate of nearly
100% after interacting with Au-Au/IrO2@Cu (PABA) for 3.5
h. Furthermore, the reactor also exhibits excellent antibacterial
activity, so as to achieve a complete bactericidal effect to Staphylococcus aureus and Escherichia
coli at a concentration of 12.5 μg mL–1.