jp407164q_si_001.pdf (183.89 kB)
Assembled Hemoglobin and Catalase Nanotubes for the Treatment of Oxidative Stress
journal contribution
posted on 2013-09-26, 00:00 authored by Bo Chen, Yi Jia, Jie Zhao, Hong Li, Weiguang Dong, Junbai LiHemoglobin-based
nanotubes for the treatment of oxidative stress
are successfully fabricated via covalent layer-by-layer assembly.
Catalase, the most efficient enzyme for scavenging the hydrogen peroxide
(H2O2), is embedded into the inner pores of
the hemoglobin/dialdehyde heparin (Hb/DHP)5 nanotubes via
covalent bonding to protect the delicate enzyme from inactivation
after the removal of the polycarbonate (PC) template. Dialdehyde heparin
(DHP) as wall components is a cross-linker. The biocompatibility and
capabilities of the nanotubes to protect the cells under oxidative
stress are characterized with the Cell Counting Kit-8 (CCK-8). The
scavenging capabilities of four kinds of autofluorescent nanotubes
are tested through the reaction with H2O2 by
the measurement of the UV–vis absorbance at 240 nm. All the
experimental results show that the assembled hemoglobin-based nanotubes
possess H2O2-scavenging capacity as well as
biocompatibility, indicating the potential application in the treatment
of oxidative stress.