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Ascorbic Acid-Loaded Apoferritin-Assisted Carbon Dot-MnO2 Nanocomposites for the Selective and Sensitive Detection of Trypsin

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journal contribution
posted on 2018-08-24, 00:00 authored by Qingqing Tan, Ruirui Zhang, Weisu Kong, Fengli Qu, Limin Lu
A novel “turn-on” fluorescent trypsin detection platform dependant on carbon dot-MnO2 (CD-MnO2) nanocomposites and ascorbic acid-loaded apoferritin (APOAA) was fabricated. The detection mechanism relied on trypsin-catalyzed enzymolysis of APOAA, which released ascorbic acid (AA) as a reducing agent to disintegrate the MnO2 nanosheets, causing the recovery of the fluorescence of CDs. An excellent performance and high sensitivity of trypsin determination were observed with a detection limit (LOD) of 0.3411 ng/mL. This work provides us with a unique strategy for trypsin detection in human serum samples, which reveals the potential applications in clinical detection.