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Artificial Spectrin Shells Reconstituted on Giant Vesicles

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journal contribution
posted on 16.12.2015, 21:31 by Iván López-Montero, Ruddi Rodríguez-García, Francisco Monroy
In the experimental approach to a synthetic minimal cell, the membrane compartment is a main component. Lipid vesicles represent the natural host for the artificial reconstruction of a cytomimetic membrane skeleton able to support mechanical function. Using the membrane component of human erythroid cells, we have reconstructed a membrane shell composed of a spectrin skeleton and fed by ATP. The structural and mechanical analysis reveals this spectrin skeleton as topological network supporting mechanical rigidity. Such an artificial shell would define a membrane compartment mechanically stable under physiological conditions.