ja8b13816_si_001.pdf (1.44 MB)
Artificial Signal Feedback Network Mimicking Cellular Adaptivity
journal contribution
posted on 2019-04-03, 00:00 authored by Hui Liu, Qiuxia Yang, Ruizi Peng, Hailan Kuai, Yifan Lyu, Xiaoshu Pan, Qiaoling Liu, Weihong TanInspired
by this elegant system of cellular adaptivity, we herein
report the rational design of a dynamic artificial adaptive system
able to sense and respond to environmental stresses in a unique sense-and-respond
mode. Utilizing DNA nanotechnology, we constructed an artificial signal
feedback network and anchored it to the surface membrane of a model
giant membrane vesicle (GMV) protocell. Such a system would need to
both senses incoming stimuli and emit a feedback response to eliminate
the stimuli. To accomplish this mechanistically, our DNA-based artificial
signal system, hereinafter termed DASsys, was equipped with a DNA
trigger-induced DNA polymer formation and dissociation machinery.
Thus, through a sequential cascade of stimulus-induced DNA strand
displacement, DASsys could effectively sense and respond to incoming
stimuli. Then, by eliminating the stimulus, the membrane surface would
return to its initial state, realizing the formation of a cyclical
feedback mechanism. Overall, our strategy opens up a route to the
construction of artificial signaling system capable of maintaining
homeostasis in the cellular micromilieu, and addresses important emerging
challenges in bioinspired engineering.
History
Usage metrics
Categories
Keywords
signal systemmodel giant membrane vesiclefeedback responseArtificial Signal Feedback Network Mimicking Cellular Adaptivitysequential cascademembrane surfacestimulus-induced DNA strand displacementUtilizing DNA nanotechnologybioinspired engineeringDNA trigger-induced DNA polymer formationfeedback mechanismsense-and-respond modeadaptive systemGMVdissociation machineryDASsysignal feedback networksurface membranestimuli
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC