Array-Based Sensing of Proteins Using Conjugated Polymers
journal contributionposted on 15.08.2007, 00:00 authored by Oscar R. Miranda, Chang-Cheng You, Ronnie Phillips, Ik-Bum Kim, Partha S. Ghosh, Uwe H. F. Bunz, Vincent M. Rotello
A sensor array comprising six functionalized poly(p-phenyleneethynylene) polymers was constructed and used to detect and identify protein analytes. The presence of proteins alters the fluorescence properties of the polymers, generating a distinct fluorescence response pattern for a given protein. Linear discriminant analyses accurately differentiate the patterns of 17 proteins at nano- to micromolar concentrations. An identification accuracy of 97% was obtained on the basis of the tests with 68 unknown protein samples from the training set.