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Aptamer-Functionalized Gold Nanostars for on-Demand Delivery of Anticancer Therapeutics
journal contribution
posted on 2020-06-29, 09:29 authored by Dalin Wu, Pei Zhao, Lei Wu, Lihua Lin, Guoyi Yu, Linlin Xu, Jun YueGold nanostars (AuNS)
are promising carriers for targeted delivery
of therapeutic oligonucleotides, but their potential in fabricating
an on-demand drug release system in a facile and robust way remains
to be explored. In this paper, we used a model aptamer (HApt), acting
not only as a target ligand but also as a natural thermal-responsive
material, to decorate AuNS. The prepared gold nanoconstruct, HApt@AuNS,
displayed stoichiometric loading capacity of the anthracycline drug
doxorubicin (Dox). The on-demand drug release was realized by illuminating
nanoconstructs with near-infrared (NIR) light. Furthermore, a higher
degree of Dox release from the nanoconstructs was achieved in an acidic
environment, compared to neutral conditions. The in vitro experiments showed that Dox-intercalation did not affect the cell
uptake efficiency of HApt@AuNS, which could enter cells through clathrin-mediated
endocytosis and microtubule-dependent active transport to lysosomes.
Dox-loaded HApt@AuNS exhibited intracellular on-demand drug release
and enhanced toxicity against cancer cells by NIR-irradiation.
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gold nanoconstructDox releasethermal-responsive materialclathrin-mediated endocytosistarget ligandon-demand drug releasecancer cellsHApton-demand drug release systemAuNSAnticancer Therapeutics Gold nanostarson-Demand Deliveryanthracycline drug doxorubicinmodel aptamercell uptake efficiencyacidic environmentintracellular on-demand drug releaseNIRAptamer-Functionalized Gold Nanostarsstoichiometric loading capacity
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