Application of Long Wavelength Ultraviolet Radiation for Modification and Patterning of Protein-Repelling Monolayers
journal contributionposted on 19.02.2016, 16:33 by Y. L. Jeyachandran, Theresa Weber, Andreas Terfort, Michael Zharnikov
The applicability of long wavelength (390 nm) ultraviolet (UV) light for controlled modification of protein-repelling oligo(ethylene glycol) terminated alkanethiolate (OEG-AT) monolayers on gold was studied. It was found that these films can be modified in a controlled way even at such a long wavelength, which offers versatile strategies for the design of mixed monomolecular films and chemical lithography. Using the UV treatment, protein affinity of the OEG-AT film could be either directly adjusted for nonspecific adsorption or, in combination with subsequent exchange reactions for molecules bearing a specific receptor, precisely tuned for specific adsorption. The parameters of both procedures were determined, and the results were compared with analogous data for UV light with shorter wavelengths. It occurred that 390 nm UV light is especially well suited for the fabrication of specific protein patterns. In addition, because of the long wavelength, well-developed standard optics and commercial patterning strategies can be potentially utilized.