Antiviral Effect of Epigallocatechin Gallate on Enterovirus 71
journal contributionposted on 22.07.2009, 00:00 by Hung-Yao Ho, Mei-Ling Cheng, Shiue-Fen Weng, Yann-Lii Leu, Daniel Tsun-Yee Chiu
Oxidative stress is known to be a determinant of a host’s susceptibility to pathogens. Natural compounds with antioxidant activity may provide a preventive measure against infection. Tea polyphenols were evaluated for their ability to inhibit enterovirus 71 (EV71) replication in Vero cell culture. Among the polyphenolic compounds tested, epigallocatechin gallate (EGCG) and gallocatechin gallate (GCG) potently inhibited replication of EV71. EGCG and GCG reduced the titer of infectious progeny virus by 95%. Quantitative RT-PCR analysis also revealed that EGCG suppressed replication of genomic RNA. It was accompanied by an increased cytoprotective effect. EGCG and GCG caused 5-fold increase in the viability of EV71-infected cells. The viral inhibitory effect correlated well with the antioxidant capacity of polyphenol. Mechanistically, EV71 infection led to increased oxidative stress, as shown by increased dichlorofluorescein and MitoSOX Red fluorescence. Upon EGCG treatment, reactive oxygen species (ROS) generation was significantly reduced. Consistent with this, EV71 replication was enhanced in glucose-6-phosphate dehydrogenase deficient cells, and such enhancement was largely reversed by EGCG. These findings suggest that EGCG may suppress viral replication via modulation of cellular redox milieu.
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EV 71 replicationEGCG treatmententerovirus 71EV 71 infectionMitoSOX Red fluorescenceantioxidant capacityprogeny virusEnterovirus 71 Oxidative stressredox milieugallocatechin gallateEV 71. EGCGcytoprotective effectROSantioxidant activitygenomic RNAreactive oxygen speciesVero cell cultureoxidative stressAntiviral Effecttea polyphenolsEpigallocatechin Gallateepigallocatechin gallatepolyphenolic compoundsNatural compounds