An Optimized
Two-Dimensional Quantitative Nuclear
Magnetic Resonance Strategy for the Rapid Quantitation of Diester-Type
C19-Diterpenoid Alkaloids from Aconitum carmichaelii
posted on 2023-05-20, 12:43authored byQiao Lin, Chunwang Meng, Jie Liu, Fei Liu, Qinmei Zhou, Juan Liu, Cheng Peng, Liang Xiong
With the development of nuclear magnetic resonance (NMR)
spectrometers
and probes, two-dimensional quantitative nuclear magnetic resonance
(2D qNMR) technology with a high signal resolution and great application
potential has become increasingly accessible for the quantitation
of complex mixtures. However, the requirement that the relaxation
recovery time be equal to at least five times T1 (longitudinal relaxation time) makes it difficult for 2D
qNMR to simultaneously achieve high quantitative accuracy and high
data acquisition efficiency. By comprehensively using relaxation optimization
and nonuniform sampling, we successfully established an optimized
2D qNMR strategy for HSQC experiments at the half-hour level and then
accurately quantified the diester-type C19-diterpenoid
alkaloids in Aconitum carmichaelii.
The optimized strategy had the advantages of high efficiency, high
accuracy, good reproducibility, and low cost and thus could serve
as a reference to optimize 2D qNMR experiments for quantitative analysis
of natural products, metabolites, and other complex mixtures.