ac0c01623_si_001.pdf (662.18 kB)
An Endoplasmic Reticulum-Targeted Ratiometric Fluorescent Probe for the Sensing of Hydrogen Sulfide in Living Cells and Zebrafish
journal contribution
posted on 2020-07-02, 19:05 authored by Wei Shu, Shunping Zang, Chong Wang, Mengxu Gao, Jing Jing, Xiaoling ZhangHydrogen
sulfide (H2S) is an endogenous gaseous signaling
molecule in many physiological processes. Relevant investigations
indicated that H2S plays a cytoprotective effect under
endoplasmic reticulum stress. Currently, it is still a challenge to
design effective methods for ratio detection of endoplasmic reticulum
H2S. Herein we are the first to construct a ratiometric
near-infrared fluorescent probe (M-H2S) for sensing H2S in the endoplasmic reticulum. M-H2S has high
selectivity and sensitivity toward H2S (LOD = 39.1 nM).
Additionally, M-H2S possessed an excellent endoplasmic
reticulum targeting ability. Moreover, M-H2S was successfully
utilized to visualize exogenous/endogenous H2S in HeLa
cells and zebrafish. Particularly important, endogenously produced
H2S was observed under endoplasmic reticulum stress induced
by tunicamycin.