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Download fileAn Acetylcholinesterase-Functionalized Biosensor for Sensitive Detection of Organophosphorus Pesticides Based on Solution-Gated Graphene Transistors
journal contribution
posted on 2021-08-05, 14:36 authored by Rongrong Wang, Yuhong Wang, Hao Qu, Lei ZhengIn
this work, a highly sensitive and selective organophosphorus
pesticide (OP) biosensor based on a solution-gated graphene transistor
(SGGT) has been successfully constructed. The electrochemical performance
of the device is improved by immobilization of acetylcholinesterase
(AChE) on the gold gate electrode with chitosan (CHIT) for detection
of OPs. The AChE/CHIT-functionalized SGGT sensor shows an ultralow
detection limit of 10 nM for OPs and a wide linear range from 300
nM to 3 μM. We also proved that the SGGT-based OP sensor has
good selectivity and recovery performance for efficient evaluation
of the level of OPs in actual rice samples. This sensor provides a
promising detection platform for rapid and sensitive analysis of OPs
in real samples with complex components and can be readily applied
to many other important analytes for agricultural and food safety
using the corresponding enzymes.
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Keywords
gold gate electrodeultralow detection limitSGGT-based OP sensor300 nMCHITSensitive Detectionorganophosphorus pesticiderecovery performanceOrganophosphorus Pesticidessolution-gated graphene transistordetection platform10 nMelectrochemical performanceSolution-Gated Graphene Transistors3 μ MAcetylcholinesterase-Functionalized...food safetyrice samples