posted on 2017-03-29, 17:49authored byYousef Dashti, Tanja Grkovic, Usama Ramadan Abdelmohsen, Ute Hentschel, Ronald J. Quinn
The metabolite profiles of three
sponge-derived actinomycetes,
namely, Micromonospora sp. RV43, Rhodococcus sp. RV157, and Actinokineospora sp. EG49 were investigated
after elicitation with N-acetyl-d-glucosamine. 1H NMR fingerprint methodology was utilized to study the differences
in the metabolic profiles of the bacterial extracts before and after
elicitation. Our study found that the addition of N-acetyl-d-glucosamine modified the secondary metabolite
profiles of the three investigated actinomycete isolates. N-Acetyl-d-glucosamine induced the production of
3-formylindole (11) and guaymasol (12) in Micromonospora sp. RV43, the siderophore bacillibactin 16, and surfactin antibiotic 17 in Rhodococcus sp. RV157 and increased the production of minor metabolites actinosporins
E–H (21–24) in Actinokineospora sp. EG49. These results highlight the use of NMR fingerprinting
to detect changes in metabolism following addition of N-acetyl-d-glucosamine. N-Acetyl-d-glucosamine was shown to have multiple effects including suppression
of metabolites, induction of new metabolites, and increased production
of minor compounds.