posted on 2022-06-13, 20:13authored byMihebai Yilimulati, Lang Zhou, Dmitry Shevela, Shujuan Zhang
The regulation of photosynthetic
machinery with a nonoxidative
approach is a powerful but challenging strategy for the selective
inhibition of bloom-forming cyanobacteria. Acetylacetone (AA) was
recently found to be a target-selective cyanocide for Microcystis aeruginosa, but the cause and effect
in the studied system are still unclear. By recording of the chemical
fingerprints of the cells at two treatment intervals (12 and 72 h
with 0.1 mM AA) with omics assays, the molecular mechanism of AA in
inactivating Microcystis aeruginosa was elucidated. The results clearly reveal the effect of AA on ferredoxin
and the consequent effects on the physiological and biochemical processes
of Microcystis aeruginosa. In addition
to its role as an electron acceptor of photosystem I, ferredoxin plays
pivotal roles in the assimilation of nitrogen in cyanobacterial cells.
The effect of AA on ferredoxin and on nonheme iron of photosystem
II first cut off the photosynthetic electron transfer flow and then
interrupted the synthesis of adenosine triphosphate (ATP) and reduced
nicotinamide adenine dinucleotide phosphate (NADPH), which ultimately
might affect carbon fixation and nitrogen assimilation metabolisms.
The results here provide missing pieces in the current knowledge on
the selective inhibition of cyanobacteria, which should shed light
on the better control of harmful blooms.