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A Visual Leaf Zymography Technique for the In Situ Examination of Plant Enzyme Activity under the Stress of Environmental Pollution
journal contribution
posted on 2020-09-02, 05:43 authored by Yu-Xi Feng, Xin Chen, Yan-Wen Li, Hai-Ming Zhao, Lei Xiang, Hui Li, Quan-Ying Cai, Nai-Xian Feng, Ce-Hui Mo, Ming-Hung WongThis
study established a high-efficiency fluorescence quenching
approach for the in situ visualization and modeling
of the spatial distribution of xylanase, β-glucosidase, and
phosphatase activities in plant leaves under pollution stress (namely,
the leaf zymography technique, LZT). In the LZT, a membrane saturated
with an enzyme-specific fluorescent substrate on the leaf surface
was incubated and the fluorescence image generated on the membrane
under ultraviolet light was recorded. An image-based modeling method
for restoring the morphological traits of the true image by reducing
noise was developed to ensure the accurate estimation of enzyme activities.
The LZT could simultaneously measure 48 samples within 2 h, with good
reproducibility. The results obtained by the LZT were comparable to
those obtained by a conventional biochemical analysis method and presented
low-cost and convenient advantages. This paper explains the theoretical
basis required to investigate the realistic application of the LZT
for assessing ecotoxicity in large-scale monitoring.
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Visual Leaf Zymography Techniquefluorescence image48 samplesβ- glucosidaseLZTenzyme activitiesphosphatase activitiesimage-based modeling methodmembraneleaf zymography techniqueleaf surface2 hPlant Enzyme Activityanalysis methodEnvironmental Pollutionpollution stresshigh-efficiency fluorescence quench...Situ Examination
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