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Download fileA Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns
journal contribution
posted on 30.06.2020, 20:43 authored by Kenneth
P. Garrard, Måns Ekelöf, Sitora Khodjaniyazova, M. Caleb Bagley, David C. MuddimanA vision-system
driven platform, RastirX, has been constructed
for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns.
The user identifies a region of interest (ROI) by drawing on a live
video image of the sample with the computer mouse. Motion commands
are automatically generated to move the sample to acquire scan data
for the pixels in the ROI. Synchronization of sample stage motion
with laser firing and mass spectrometer (MS) scan acquisition is fully
automated. RastirX saves a co-registered optical image and the scan
location information needed to convert raw MS data into imzML format.
Imaging an arbitrarily shaped ROI instead of the minimal enclosing
rectangle reduces contamination from off-sample material and significantly
reduces acquisition time.