posted on 2012-11-14, 00:00authored byQuanjuan Zhang, Zhichuan Zhu, Yongli Zheng, Jiagao Cheng, Na Zhang, Yi-Tao Long, Jing Zheng, Xuhong Qian, Youjun Yang
A novel fluorescent probe for peroxynitrite, PN600,
was rationally designed on the basis of a unique fluorophore assembly
approach. PN600 is a green-emitting coumarin derivative.
Upon oxidation by peroxynitrite, PN600 is transformed into
a highly fluorescent red-emitting resorufin derivative via an orange-emitting
intermediate. This three-channel signaling capability enables PN600 to differentiate peroxynitrite from other reactive oxygen
and nitrogen species, including hypochlorite and hydroxyl radical.
Moreover, PN600 is membrane-permeable and compatible with
common TRITC filter sets and displays low cytotoxicity. Therefore,
PN600 is a promising candidate for in vitro peroxynitrite
imaging.