American Chemical Society
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A Three-Channel Fluorescent Probe That Distinguishes Peroxynitrite from Hypochlorite

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journal contribution
posted on 2012-11-14, 00:00 authored by Quanjuan Zhang, Zhichuan Zhu, Yongli Zheng, Jiagao Cheng, Na Zhang, Yi-Tao Long, Jing Zheng, Xuhong Qian, Youjun Yang
A novel fluorescent probe for peroxynitrite, PN600, was rationally designed on the basis of a unique fluorophore assembly approach. PN600 is a green-emitting coumarin derivative. Upon oxidation by peroxynitrite, PN600 is transformed into a highly fluorescent red-emitting resorufin derivative via an orange-emitting intermediate. This three-channel signaling capability enables PN600 to differentiate peroxynitrite from other reactive oxygen and nitrogen species, including hypochlorite and hydroxyl radical. Moreover, PN600 is membrane-permeable and compatible with common TRITC filter sets and displays low cytotoxicity. Therefore, PN600 is a promising candidate for in vitro peroxynitrite imaging.