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A Switchable Surface Enables Visualization of Single DNA Hybridization Events with Atomic Force Microscopy

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journal contribution
posted on 01.05.2013, 00:00 by Gary R. Abel, Eric A. Josephs, Norman Luong, Tao Ye
Here we describe a novel surface that enables direct visualization of the hybridization of single DNA molecules with an unprecedented resolution using atomic force microscopy. The surface consists of single-stranded DNA probes that are covalently anchored to a self-assembled monolayer. The surface satisfies the contradictory requirements for high-resolution imaging and hybridization by switching the DNA-surface interaction between a strong state and a weak state. Our approach opens up unique opportunities in elucidating hybridization at the molecular scale.