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A Straightforward Method for the Colorimetric Detection of Endogenous Biological Cyanide

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journal contribution
posted on 2009-11-15, 00:00 authored by Christine Männel-Croisé, Benjamin Probst, Felix Zelder
Corrin-based chemosensors allow the rapid and selective colorimetric detection of endogenous biological cyanide. The color change from orange to violet can be easily observed with the “naked eye” (Δλmax = 51 nm). The methodology works directly in the biological matrix without time-consuming sample pretreatment and the use of special reaction conditions. It was possible to study the enzymatic release of cyanide from its biological precursor linamarin with diffuse reflectance UV−visible (DRUV−vis) spectroscopy on a freshly prepared biological surface. These experiments were accompanied by stopped-flow kinetic measurements under homogeneous conditions. Detection in the biological sample is based on the selective coordination of cyanide to the metal-based chemosensor as proven by UV−vis and 1H NMR spectroscopy. Examples of applications during food manufacturing are given.

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