American Chemical Society
ic8b00620_si_001.pdf (7 MB)

A Series of Zn(II) Terpyridine-Based Nitrate Complexes as Two-Photon Fluorescent Probe for Identifying Apoptotic and Living Cells via Subcellular Immigration

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journal contribution
posted on 2018-06-19, 00:00 authored by Dandan Liu, Mingzhu Zhang, Wei Du, Lei Hu, Fei Li, Xiaohe Tian, Aidong Wang, Qiong Zhang, Zhongping Zhang, Jieying Wu, Yupeng Tian
Two-photon active probe to label apoptotic cells plays a significant role in biological systems. However, discrimination of live/apoptotic cells at subcellular level under microscopy remains unachieved. Here, three novel Zn­(II) terpyridine-based nitrate complexes (C1C3) containing different pull/push units were designed. The structures of the ligands and their corresponding Zn­(II) complexes were confirmed by single-crystal X-ray diffraction analysis. On the basis of the comprehensive comparison, C3 had a suitable two-photon absorption cross section in the near-infrared wavelength and good biocompatibility. Under two-photon confocal microscopy and transmission electron microscopy, it is found that C3 could target mitochondria in living cells but immigrate into the nucleolus during the apoptotic process. This dual-functional probe (C3) not only offers a valuable image tool but also acts as an indicator for cell mortality at subcellular level in a real-time manner.