nn9b10144_si_001.pdf (4.71 MB)
A Self-Evaluating Photothermal Therapeutic Nanoparticle
journal contribution
posted on 2020-07-29, 10:43 authored by Yanfang Wang, Wei Du, Tong Zhang, Yu Zhu, Yanhan Ni, Chenchen Wang, Fatima Maria Sierra Raya, Liwei Zou, Longsheng Wang, Gaolin LiangToday,
tumor therapy and its therapeutic efficiency evaluation
are conducted separately, and current imaging techniques cannot evaluate
tumor-therapeutic effects in real time. Therefore, it is of great
importance to develop highly efficient theranostic strategies which
are able to evaluate their tumor-therapeutic effects in real time.
In this work, by rational design of a small molecular near-infrared
probe Cys(StBu)-Asp-Glu-Val-Asp-Lys(Cypate)-CBT (Cy-CBT) and using a CBT-Cys click condensation reaction, we facilely prepare
an intelligent nanoparticle Cy-CBT-NP which is able to
evaluate its photothermal therapy (PTT) efficiency on tumors by fluorescence
“Turn-On”. Fluorescence of Cy-CBT-NP is
quenched and photothermal responsive. Upon caspase 3 (Casp3) cleavage
of its DEVD substrates, Cy-CBT-NP disassembles to turn
the fluorescence “On”, which in turn evaluates the PTT
efficiency of the nanoparticle on cells and tumors in real time. We
envision that our smart strategy could be applied for PTT and real-time
evaluation of the therapeutic efficiency of solid tumors in the near
future.