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Download fileA Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation
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posted on 2021-02-15, 17:33 authored by Hao Cui, Xinying Zhu, Zhaoguang Wang, Jianping Fang, Tao YuanA structurally defined konjac glucomannan
oligosaccharide (KGMOS)
with a relatively high molecular weight and narrow molecular weight
distribution (molecular weight ranging from 3000 to 4000 Da, degree
of polymerization (dp) 8–11) was prepared from native konjac
glucomannan (KGM), and the beneficial effects and molecular mechanisms
of KGMOS on colonic functions were investigated in C57BL/6 mice. The
results are the first to reveal that KGMOS regulated intestinal microflora
composition to facilitate the production of colonic butyrate. Elevated
butyrate production further increased the acetylation of histone proteins
H3 and H4 and thus enhanced the transcription of the major colonic
mucin gene Muc2 and the secretion of mucin elements,
which represents a new molecular mechanism of KGM oligosaccharide
consumption. The findings indicate that KGM oligosaccharides with
specific molecular sizes have highly desirable functional properties
and potentially could improve gut health by promoting the barrier
function of the colonic mucosa.
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KGMOSkonjac glucomannan oligosaccharideweight distributionKGM oligosaccharide consumptionC 57BL micemucin elementsAmorphophallus konjac4000 Damicroflora compositionhistone proteins H 3gut healthmechanismH 4Histone Protein H 3H 4 Acetylationkonjac glucomannanElevated butyrate productionbarrier functionEnhancing Butyrate Productionmucin gene Muc 2KGM oligosaccharidesGlucomannan OligosaccharideColonic Mucosal Barrier Function