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A Promising Family of Fluorescent Water-Soluble aza-BODIPY Dyes for in Vivo Molecular Imaging

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journal contribution
posted on 07.01.2019, 00:00 by Jacques Pliquett, Adrien Dubois, Cindy Racoeur, Nesrine Mabrouk, Souheila Amor, Robin Lescure, Ali Bettaïeb, Bertrand Collin, Claire Bernhard, Franck Denat, Pierre Simon Bellaye, Catherine Paul, Ewen Bodio, Christine Goze
A new family of water-soluble and bioconjugatable aza-BODIPY fluorophores was designed and synthesized using a boron- functionalization strategy. These dissymmetric bis-ammonium aza-BODIPY dyes present optimal properties for a fluorescent probe; i.e., they are highly water-soluble, very stable in physiological medium; they do not aggregate in PBS, possess high quantum yield; and finally, they can be easily bioconjugated to antibodies. Preliminary in vitro and in vivo studies were performed for one of these fluorophores to image PD-L1 (Programmed Death-Ligand 1), highlighting the high potential of these new probes for future in vivo optical imaging studies.