A Potent, Water-Soluble and Photoinducible DNA Cross-Linking Agent

Wang, Ping; Liu, Renpeng; Wu, Xiaojun; Ma, Hongjuan; Cao, Xiaoping; Zhou, Ping; Zhang, Jiangye; Weng, Xiaocheng; Zhang, Xiao-Lian; Qi, Jun; Zhou, Xiang; Weng, Linhong

doi:10.1021/ja029040o.s001

ja029040o_si_001.pdf (248.4 kB)

A Potent, Water-Soluble and Photoinducible DNA Cross-Linking Agent

journal contribution

posted on 2003-01-09, 00:00 authored by Ping Wang, Renpeng Liu, Xiaojun Wu, Hongjuan Ma, Xiaoping Cao, Ping Zhou, Jiangye Zhang, Xiaocheng Weng, Xiao-Lian Zhang, Jun Qi, Xiang Zhou, Linhong Weng

Water-soluble DNA cross-linking phenol and biphenol derivatives 3 and 6 have been synthesized by a Mannich reaction. Both of them can cross-link DNA by photoactivation using visible light (wavelength > 400 nm). Compound 6 can cross-link DNA at pH 5.0 and 7.7, whereas no cross-link was observed at pH 10.0. Density functional theory (DFT) calculation indicated that 6 displays a twist structure. Therefore, it could bind to DNA naturally and has potent property to cross-link DNA after photoactivation.