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A Photolabile Linker for the Mild and Selective Cleavage of Enriched Biomolecules from Solid Support

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journal contribution
posted on 06.11.2009, 00:00 by Ronald Orth, Stephan A. Sieber
Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.