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A Novel Immunostimulator, N2-[α-O-Benzyl-N-(acetylmuramyl)-l-alanyl-d- isoglutaminyl]-N6-trans-(m-nitrocinnamoyl)-l-lysine, and Its Adjuvancy on the Hepatitis B Surface Antigen
journal contribution
posted on 2005-08-11, 00:00 authored by Hong-Zhen Yang, Song Xu, Xue-Yan Liao, Suo-De Zhang, Zheng-Lun Liang, Bai-He Liu, Jin-Ye Bai, Chao Jiang, Jian Ding, Gui-Fang Cheng, Gang LiuN2-[α-O-Benzyl-N-(acetylmuramyl)-l-alanyl-d-isoglutaminyl]-N6-trans-(m-nitrocinnamoyl)-l-lysine (muramyl dipeptide C, or MDP-C) has been synthesized as a novel, nonspecific
immunomodulator. The present study shows that MDP-C induces strong cytolytic activity by
macrophages on P388 leukemia cells and cytotoxic activity by cytotoxic T lymphocytes (CTLs)
on P815 mastocytoma cells. Our results also indicate that MDP-C is an effective stimulator
for production of interleukin-2 and interleukin-12 by murine bone morrow derived dendritic
cells (BMDCs) and production of interferon-γ by CTLs. Additionally, MDP-C increases the
expression levels of several surface molecules, including CD11c, MHC class I, and intercellular
adhesion molecule-1 in BMDCs. Moreover, MDP-C remarkably enhances the immune system's
responsiveness to hepatitis B surface antigen (HBsAg) in hepatitis B virus transgenic mice for
both antibody production and specific HBsAg T-cell responses ex vivo. Our results indicate
that MDP-C is an apyrogenic, nonallergenic, and low-toxicity immunostimulator with great
potential for diagnostic, immunotherapeutic, and prophylactic applications in diseases such
as hepatitis B and cancers.
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intercellular adhesion moleculenitrocinnamoylantibody productionhepatitis B virushepatitis B surface antigentranNovel ImmunostimulatorinterleukinP 388 leukemia cellsBenzylHBsAgcytotoxic T lymphocytescytolytic activitymuramyl dipeptide CP 815 mastocytoma cellsCTLsurface moleculesprophylactic applicationsmurine bone morrowacetylmuramylBMDCCD 11c MHC classexpression levelshepatitis Bdendritic cellscytotoxic activity
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