A New Nanobiocatalytic System Based on Allosteric Effect with Dramatically Enhanced Enzymatic Performance
journal contributionposted on 30.01.2013, 00:00 by Liang-Bing Wang, You-Cheng Wang, Rong He, Awei Zhuang, Xiaoping Wang, Jie Zeng, J. G. Hou
We report a rational design of CaHPO4-α-amylase hybrid nanobiocatalytic system based on allosteric effect and an explanation of the increase in catalytic activity when certain enzymes are immobilized in specific nanomaterials. Employing a calcification approach in aqueous solutions, we acquired such new nanobiocatalytic systems with three different morphologies, i.e., nanoflowers, nanoplates, and parallel hexahedrons. Through studying enzymatic performance of these systems and free α-amylase with/without Ca2+, we demonstrated how two factors, allosteric regulation and morphology of the as-synthesized nanostructures, predominantly influence enzymatic activity. Benefiting from both the allosteric modulation and its hierarchical structure, CaHPO4-α-amylase hybrid nanoflowers exhibited dramatically enhanced enzymatic activity. As a bonus, the new system we devised was found to enjoy higher stability and durability than free α-amylase plus Ca2+.