A NIR Dual-Channel
Fluorescent Probe for Fluctuations
of Intracellular Polarity and H2O2 and Its Applications
for the Visualization of Inflammation and Ferroptosis
posted on 2024-02-21, 13:34authored byXing Tian, Jiayi Cheng, Lei Yang, Zhanxian Li, Mingming Yu
Hydrogen peroxide and polarity are closely related to
many physiological
activities and pathological processes. However, near-infrared fluorescent
probes that are sensitive to both H2O2 and polarity
are still scarce. Herein, we developed the first dual-channel near-infrared
fluorescent probe NBO, with an AIE effect, enabling simultaneous monitoring
of H2O2 and polarity. The probe presented high
sensitivity, high selectivity, and low detection limit for H2O2. It also had high sensitivity to polarity, independent
of pH and viscosity, with large Stokes shifts, good photostability,
and low cytotoxicity. Moreover, NBO was able to detect both endogenous
and exogenous H2O2 as well as polarity fluctuations in vivo as a method to effectively differentiate between
cancer cells and normal cells. Importantly, it also could monitor
the therapeutic effects of drugs in inflammation and iron-dead cells
and mice. Based on NIR emission, NBO could be used as an imaging tool
and a way to evaluate the therapeutic effect of drugs for inflammation
and ferroptosis.