A Luciferase-Like Monooxygenase and Flavin Reductase Pair AbmE2/AbmZ Catalyzes Baeyer–Villiger Oxidation in Neoabyssomicin Biosynthesis

Baeyer–Villiger monooxygenases (BVMOs) play important roles in the biosynthesis of natural products. We describe here in vivo gene inactivation/complementation studies as well as in vitro enzyme assays that reveal the bacterial luciferase-like monooxgenase (LLM) AbmE2 and its flavin reductase partner AbmZ as a type II BVMO pair that catalyzes BV oxidation during neoabyssomicin assembly in Streptomyces koyangensis SCSIO 5802. This study provides direct biochemical evidence of an LLM that catalyzes BV oxidation.