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A Facile Method to Synthesize Histones with Posttranslational Modification Mimics

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journal contribution
posted on 03.07.2012, 00:00 by Zhiyong U. Wang, Yane-Shih Wang, Pei-Jing Pai, William K. Russell, David H. Russell, Wenshe R. Liu
Using an evolved pyrrolysyl-tRNA synthetase-tRNAPyl pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

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