The
drug toxicity is a long-term concern in contemporary medical
research. Serious drug toxicity may cause acute liver failure or even
patient death. Currently, the pathogenesis of drug-induced liver injury
(DILI) is not entirely clear, and there is still no specific treatment
for patients with DILI. Accordingly, improving the diagnosis and treatment
level of DILI is a major challenge facing the basic and clinical research
in relevant fields. To address these, an ·OH-activated circular
dichroism (CD) and photoacoustic (PA) dual-mode nanoprobe was here
designed and synthesized. The probe was prepared using chiral d/l-cysteine and polymolybdate as raw materials to
form a nanocomplex with chiral properties (Ox-POM@d/l-Cys) based on the interaction between metal ions
and sulfhydryl groups in Ox-POM@d/l-Cys. In vitro and in vivo experimental
results have shown that the as-proposed dual-mode nanoprobe can be
used not only for CD spectral detection of Fenton’s reagent
but also for PA imaging monitoring of ·OH. More importantly,
inspired by the NIR PA properties, the Ox-POM@d/l-Cys probe was used for the first time to detect
·OH in DILI mice. This will provide very useful information for
the diagnosis and treatment of DILI disease.