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A Cell Culture Substrate with Biologically Relevant Size-Scale Topography and Compliance of the Basement Membrane
journal contribution
posted on 2015-12-17, 00:51 authored by Shaun P. Garland, Clayton T. McKee, Yow-Ren Chang, Vijay Krishna Raghunathan, Paul Russell, Christopher J. MurphyA growing
body of literature broadly documents that a wide array of fundamental
cell behaviors are modulated by the physical attributes of the cellular
microenvironment, yet in vitro assays are typically
carried out using tissue culture plastic or glass substrates that
lack the 3-dimensional topography present in vivo and have stiffness values that far exceed that of cellular and stromal
microenvironments. This work presents a method for the fabrication
of thin hydrogel films that can replicate arbitrary topographies with
a resolution of 400 nm that possess an elastic modulus of approximately
250 kPa. Material characterization including swelling behavior and
mechanics were performed and reported. Cells cultured on these surfaces
patterned with anisotropic ridges and grooves react to the biophysical
cues present and show an alignment response.
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stiffness valuestopographyhydrogel filmsmoduluattributelacksurfaceBasement MembraneACell Culture Substratearraystromal microenvironmentsalignment responsecuedocumentmethodBiologicallyCompliancematerial characterizationfabrication400 nmglass substratesTopographytopographietissue culture plasticgroove250 kPavivoanisotropic ridgesassaycell behaviors