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Download fileA Bacterial–Fungal Metaproteomic Analysis Enlightens an Intriguing Multicomponent Interaction in the Rhizosphere of Lactuca sativa
journal contribution
posted on 2012-04-06, 00:00 authored by Marino Moretti, Daniela Minerdi, Peter Gehrig, Angelo Garibaldi, Maria
Lodovica Gullino, Katharina RiedelFusarium oxysporum MSA 35 [wild-type
(WT) strain] is an antagonistic isolate that protects plants against
pathogenic Fusaria. This strain lives in association with ectosymbiotic
bacteria. When cured of the prokaryotic symbionts [cured (CU) form],
the fungus is pathogenic, causing wilt symptoms similar to those of F. oxysporum f.sp. lactucae. The aim of
this study was to understand if and how the host plant Lactuca
sativa contributes to the expression of the antagonistic/pathogenic
behaviors of MSA 35 strains. A time-course comparative analysis of
the proteomic profiles of WT and CU strains was performed. Fungal
proteins expressed during the early stages of plant-fungus interaction
were involved in stress defense, energy metabolism, and virulence
and were equally induced in both strains. In the late phase of the
interkingdom interaction, only CU strain continued the production
of virulence- and energy-related proteins. The expression analysis
of lettuce genes coding for proteins involved in resistance-related
processes corroborated proteomic data by showing that, at the beginning
of the interaction, both fungi are perceived by the plant as pathogen.
On the contrary, after 8 days, only the CU strain is able to induce
plant gene expression. For the first time, it was demonstrated that
an antagonistic F. oxysporum behaves initially as
pathogen, showing an interesting similarity with other beneficial
organisms such as mychorrizae.