posted on 2018-04-03, 00:00authored byHugues Brisset, Jean-François Briand, Raphaëlle Barry-Martinet, The Hy Duong, Pierre Frère, Frédéric Gohier, Philippe Leriche, Christine Bressy
Several alternatives
are currently investigated to prevent and
control the natural process of colonization of any seawater submerged
surfaces by marine organisms. Since few years we develop an approach
based on addressable electroactive coatings containing conducting
polymers or polymers with lateral redox groups. In this article we
describe the use of a screen-printed plate formed by 96 three-electrode
electrochemical cells to assess the potential of these electroactive
coatings to prevent the adhesion of marine bacteria. This novel platform
is intended to control and record the redox properties of the electroactive
coating in each well during the bioassay (15 h) and to allow screening
its antiadhesion activity with enough replicates to support significant
conclusions. Validation of this platform was carried out with poly(ethylenedioxythiophene)
(PEDOT) as electroactive coating obtained by electropolymerization
of EDOT monomer in artificial seawater electrolyte on the working
electrode of each electrochemical cell of the 96-well microplate.