The Importance of an Endotoxin-Free Environment during the Production of Nanoparticles Used in Medical Applications
journal contributionposted on 09.08.2006 by Helen Vallhov, Jian Qin, Sara M. Johansson, Niklas Ahlborg, Mamoun A. Muhammed, Annika Scheynius, Susanne Gabrielsson
Any type of content formally published in an academic journal, usually following a peer-review process.
We investigated the effect of spherical gold nanoparticles on immature dendritic cells (DCs). Conventionally produced nanoparticles had a maturating effect on the DCsa result of lipopolysaccharide (LPS) contamination. By modification of the production process, low-LPS particles were obtained, which had practically no effect on phenotypic maturation or cytokine production of the DCs. Our findings emphasize the importance of high purity in the production of nanoparticles, since possible contaminants may interfere with the assessment of biological/medical effects. They also highlight that nanoparticles can function as carriers of immune modulating contaminants.