jo7b03214_si_001.pdf (7.23 MB)

Synthesis and Spectroscopic Properties of Fluorinated Coumarin Lysine Derivatives

Download (7.23 MB)
journal contribution
posted on 29.03.2018 by Lakshmi Shukla, Lindon W. K. Moodie, Tomas Kindahl, Christian Hedberg
The site-selective incorporation of fluorescent amino acids into proteins has emerged as a valuable alternative to expressible protein reporters. For successful application, a robust and scalable, yet flexible, route to non-natural amino acids is required. This work describes an improved synthesis of coumarin-conjugated lysine derivatives where fluorinated variants are accessed. These analogues can be utilized at low pH and should find application probing biological processes that operate under acidic conditions.