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Synthesis and Spectroscopic Properties of Fluorinated Coumarin Lysine Derivatives

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journal contribution
posted on 29.03.2018 by Lakshmi Shukla, Lindon W. K. Moodie, Tomas Kindahl, Christian Hedberg
The site-selective incorporation of fluorescent amino acids into proteins has emerged as a valuable alternative to expressible protein reporters. For successful application, a robust and scalable, yet flexible, route to non-natural amino acids is required. This work describes an improved synthesis of coumarin-conjugated lysine derivatives where fluorinated variants are accessed. These analogues can be utilized at low pH and should find application probing biological processes that operate under acidic conditions.

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