Synthesis, Photophysical Characterization, and Enzymatic Incorporation of a Microenvironment-Sensitive Fluorescent Uridine Analog

2011-03-04T00:00:00Z (GMT) by Maroti G. Pawar Seergazhi G. Srivatsan
The synthesis of a microenvironment-sensitive base-modified fluorescent ribonucleoside analog based on a 5-(benzo[b]thiophen-2-yl)pyrimidine core, enzymatic incorporation of its corresponding triphosphate into RNA oligonucleotides, and photophysical characterization of fluorescently modified oligoribonucleotides are described.