Sequence Dependence and Direct Measurement of Crossover Isomer Distribution in Model Holliday Junctions using NMR spectroscopy†
journal contributionposted on 19.03.1996 by Göran Carlström, Walter J. Chazin
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A 32-base-pair model of the Holliday junction (HJ) intermediate in genetic recombination has been prepared and analyzed in-depth by 2D and 3D 1H NMR spectroscopy. This HJ (J2P1) corresponds to a cyclic permutation of the base pairs at the junction relative to a previously studied HJ [J2; Chen, S.-M., & Chazin, W. J. (1994) Biochemistry 33, 11453−11459], designed to probe the effect of the sequence at the n − 1 position (where n is the residue directly at the branch point) on the stacking geometry. Observation of several interbase nuclear Overhauser effects (NOEs) clearly indicates a strong preference for the isomer opposite that observed for J2, confirming the dependence of stacking isomer preference on the sequence at the junction. As for other model HJs studied, a small equilibrium distribution of the alternate isomer could be identified. A sample of J2P1 was prepared with a single 15N-labeled thymine residue at the branch point. 1D 15N-filtered 1H-detected experiments on this sample at low temperature give strong support for the co-existence of the two stacking isomers and provide a much more direct and accurate measure of the crossover isomer distribution. The comparative analysis of our immobile HJs and a model cruciform structure [Pikkemaat, J. A., van den Elst, H., van Boom, J. H., & Altona, C. (1994) Biochemistry 33, 14896−14907] sheds new light on the issue of the relevance of crossover isomer preference in vivo.