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Quantitative Characterization of Nanoparticles in Blood by Transmission Electron Microscopy with a Window-Type Microchip Nanopipet

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journal contribution
posted on 07.08.2012 by Lin-Ai Tai, Yu-Ting Kang, Yu-Ching Chen, Yu-Chao Wang, Yu-Jing Wang, Yu-Ting Wu, Kuo-Liang Liu, Chiu-Yen Wang, Yu-Feng Ko, Ching-Ya Chen, Nai-Chun Huang, Jen-Kun Chen, Yong-Fen Hsieh, Tri-Rung Yew, Chung-Shi Yang
Transmission electron microscopy (TEM) is a unique and powerful tool for observation of nanoparticles. However, due to the uneven spatial distribution of particles conventionally dried on copper grids, TEM is rarely employed to evaluate the spatial distribution of nanoparticles in aqueous solutions. Here, we present a microchip nanopipet with a narrow chamber width for sorting nanoparticles from blood and preventing the aggregation of the particles during the drying process, enabling quantitative analysis of their aggregation/agglomeration states and the particle concentration in aqueous solutions. This microchip is adaptable to all commercial TEM holders. Such a nanopipet proves to be a simple and convenient sampling device for TEM image-based quantitative characterization.